Figure 2 | Scientific Reports

Figure 2

From: Β-blockers treatment of cardiac surgery patients enhances isolation and improves phenotype of cardiosphere-derived cells

Figure 2

Gene expression levels and immunofluorescence phenotype of CDCs from β-blocker (BB) and non-β-blocker (NBB) patients.

BB-CDCs displayed significantly higher expression levels of the cardiac specific genes MHC and Nkx2.5 (a) compared to NBB (NBB n = 4, BB n = 8). Representative CDC immunofluorescence images (b) showing a higher Nkx2.5 nuclear expression in BB cells, consistently with the qRTPCR results. Albeit no differences between BB and NBB were detectable in its expression level, intracellular staining for SMA displayed significant differences at morphological and cytoskeletal levels between BB and NBB (b). ADRB1 was detectable in both BB and NBB-CDCs (b). *P < 0.05. Scale bars = 100 μm.

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