Figure 1 | Scientific Reports

Figure 1

From: GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties

Figure 1

The expression profile of growth factor genes by feeder cells and SSCs.

(a) The morphology of SSC clumps (upper panel), monolayer of STO cells (middle panel) and MEF cells (bottom panel) in culture dish, scale bar = 50 μm; (b) The semi-quantatitive PCR amplification for gene expression; mouse mRNA was extracted from SSCs, STO and MEF cells, and the β-actin gene served as a control. The experiments were repeated three times and the representative results were demonstrated in the figure.

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