Figure 3 | Scientific Reports

Figure 3

From: GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties

Figure 3

SSC propagation on a GDNF-secreting STO feeder layer.

(a) A culture of a line of SSCs harvested from 6–8 days old C57BL/6 mouse testes. The morphology of SSC clumps formed at Day 1, 7 and Day 90. Scale bar = 50 μm; (b) The growth curve calculated from a line of SSCs (combined) supported by GDNF-expressing STO feeders with the addition of 20 ng/ml GDNF, 1 ng/ml FGF2 and 150 ng/ml GFRA1 (GDNF-OE STO with GDNF, GFRA1, FGF2), a line by GDNF-expressing STO feeders with 1ng/ml FGF2 (GDNF-OE STO with FGF2), and a line by normal STO feeders with GDNF, GFRA1, FGF2 (STO with GDNF, GFRA1, FGF2). The 25-day culture data were collected through three wells of each individual line of SSCs, the error bars indicate the means ± SD.

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