Figure 4 | Scientific Reports

Figure 4

From: GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties

Figure 4

Mouse SSC transplantation.

(a) A culture of testis SSCs harvested from 6–8 days old ROSAmT/mG(129 × C57BL/6 background) mice, a two-colour fluorescent Cre reporter allele allowing the strong and persistent expression of RFP driven by the ACTB promoter before Cre-induction. The SSC clumps therefore expressed cell membrane-localised red fluorescence. Scale bar = 50 μm; (b) Extensive colonies were observed by the transplantation of the ROSAmT/mG mouse SSCs into 129 × C57BL/6 recipient mouse testes(upper panel), which is further visualised by regeneration fragments of spermatogenesis in the individual seminiferous tubule (lower panel). Scale bar = 1 mm; (c)Compared to the histology of paraffin sections from the busulfan-treated mouse testes, normal spermatogenic waves were found in the recipient testes after transplantation. Scale bar = 50 μm.

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