Figure 1
UBA52 is required for embryonic development.
(A) Diagram of human ubiquitin genes and their chromosomal locations. (B) Schematic representation of the gene-targeting construct and screening strategy. Southern blots of EcoRI- and PstI-digested genomic DNA from embryonic stem cells showing the targeted allele. The probe was specific for exon 1. (C) Polymerase chain reaction (PCR) analysis of genomic DNA obtained from the tails of wild-type (Uba52+/+) and mutant (Uba52tm1a/+) mice using the PCR primers indicated. (D) Quantitative real-time reverse-transcription PCR of Uba52 mRNA expression in tissues (kidney, spleen, small intestine, and colon) of 3-week-old mice. mRNA levels were normalized to Actb levels. Uba52+/+ (n = 8), Uba52tm1a/+ (n = 8). Error bars indicate standard deviations. N.S. indicates not significant by two-tailed Student’s t-test. (E) Numbers of surviving offspring from Uba52tm1a/+ parents. Pictures show embryonic day (E) 10.5 and E13.5 littermates. Parentheses: expected numbers based on a Mendelian ratio.
