Figure 3
SFN suppressed the CDDP-activated IL-6/STAT3 signaling.
(A,B) MGC803 and BGC823 cells were treated with 0, 0.5, 1, 2, 5, 10 or 20 μM of CDDP for 72 h. (A) ELISA analyses of secretion levels of IL-6. (B) qRT-PCR analyses of expression of IL6 (mean ± SD, n = 3). (C) MGC803 and BGC823 cells were treated with 0, 0.5, 1, or 2 μM of CDDP for 72 h. Western blot analysis of ERK, p-ERK, Akt, p-Akt, STAT3, and p-STAT3. (D,E) MGC803 and BGC823 cells were treated with 0, 2.5, 5, or 10 μM of SFN for 72 h. (D) Western blot analysis of IL-6R, STAT3, and p-STAT3. (E) qRT-PCR analyses of expressions of IL6R and STAT3 (mean ± SD, n = 3). (F) Western blot analysis was conducted after MGC803 and BGC823 cells were treated with 2 μM of CDDP, 10 μM of SFN, or their combination for 72 h. *p < 0.05 and ***p < 0.001 compared with medium control MGC803 cells. #p < 0.05 and ###p < 0.001 compared with medium control BGC823 cells.
