Figure 5

SFN suppressed IL-6R and STAT3 through activation of miR-124.

(A) MGC803 and BGC823 cells were treated with 0, 2.5, 5, or 10 μM of SFN for 72 h. The qRT-PCR analyses of miR-124 (mean ± SD, n = 3). ^**p < 0.01 and ^***p < 0.001 compared with medium control MGC803 cells. ^###p < 0.001 compared with medium control BGC823 cells. (B–D) MGC803 and BGC823 cells were transfected with con-mimic or miR-124-mimic for 12 h. (B,C) The qRT-PCR analyses of miR-124, IL6R, and STAT3 (mean ± SD, n = 3). ^***p < 0.001 compared with MGC803 cells transfected with con-mimic. ^###p < 0.001 compared with BGC823 cells transfected with con-mimic. (D) Western blot analysis of IL-6R, STAT3, and Tubulin. (E,F) MGC803 cells were transfected with anti-con or anti-miR-124 for 12 h and treated with 2 μM of CDDP, 10 μM of SFN, or their combination for 72 h. (E) qRT-PCR analyses of miR-124 (mean ± SD, n = 3). ^***p < 0.001 compared with MGC803 cells transfected with anti-con. (F) Western blot analysis of IL-6R, STAT3, p-STAT3, cyclin D, and c-Myc.