Figure 5
From: Maternal High Estradiol Exposure is Associated with Elevated Thyroxine and Pax8 in Mouse Offspring
Methylation analysis of Pax8 CpG island by bisulfite genomic sequencing PCR.
(a) Schematic representation of mouse imprinted locus, showing the relative positions of the Pax8 CpG island. Pax8 CpG island containing 15 CpG sites. The positions of exons are shown as black rectangles. CpG island is indicated as grey rectangle. (b,c) Methylation status of Pax8 CpG island and the average methylation ratio in each CpG site in thyroids of 3- (b) and 8-week-old (c). Ten clones per mouse; a total of 30 clones per group were sequenced. In the histograms, results are expressed as methylation percentage of each CpG site (n = 3 mice per group). Mean ± SE, *P < 0.05 vs. NC, x2 test. (d,e) Dnmt1, Dnmt3a, Mbd1, and Tet1 gene expression in thyroids of 3- and 8-week-old offspring (n = 6 mice per group). Mean ± SE, *P < 0.05 or **P < 0.01 vs. NC, student’s t test.
