Figure 1
Ricinoleic acid production in transgenic lines expressing the CpFAH gene.
(a) Structure of CpFAH-expression plasmid. A HindIII site for linearisation of the plasmid DNA before transformation into Chaetoceros gracilis cells is shown. Annealing sites of CpFAH-specific primers using genomic PCR are shown by arrows. (b) Expression level of CpFAH normalised by expression of the endogenous α-tubulin gene in wild-type (WT) cells and the four transgenic lines (Cp1, Cp3, Cp4, and Cp6) on d 3 °C at 20 °C. (c) Amount of ricinoleic acid (RA) in WT and transgenic cells after culturing for d 7 at 20 °C. Data in all experiments indicate mean value ± SD from three biological replicates. 14T, terminator of C. gracilis Lchr14 gene; nat1, clonNAT resistant gene, nourseothricin acetyltransferase in Streptomyces noursei; ND, not detected; PCgLhcr5, promoter of C. gracilis Lhcr5 gene; PCgACAT, promoter of C. gracilis acetyl-CoA acyltransferase gene.
