Figure 2

Temperature-dependent accumulation of ricinoleic acid in Cp4 cells.
(a,b) Time-dependent changes in ricinoleic acid (RA) content per cell (a) or as percent of total lipids (b) in Cp4 line cultured at 15 °C, 17.5 °C, 20 °C, 22.5 °C, and 25 °C. CpFAH (c) and Lhcr5 (d) gene expression in Cp4 cells cultured at 15 °C and 25 °C. Amounts of fatty acids (FAs) derived from triacylglycerol (TAG) (e) and total lipids (f ) in wild-type (WT) and Cp4 cells at 15 °C.