Figure 2

ESCs spontaneously fuse with MSCs or undergo entosis.

(a) Representative confocal micrograph of FACS-sorted eGFP⁺/mRFP⁺ cells 24 hrs after the co-culture of ESCs-mRFP and MSCs-eGFP. Internalised and degraded ESCs-mRFP within MSCs-eGFP and heterokaryon cells are indicated by white and yellow arrowheads, respectively (scale bar 10 μm). (b) FACS-sorted eGFP⁺/mRFP⁺ cells were immunostained for ß-catenin to distinguish fused (upper panel) versus entotic (lower panel) cells (scale bar 10 μm).