Figure 5
From: Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis
Hetero to synkaryon transition is mediated by mitosis.
(a) Snapshots of the time-lapse of ESCs and MSCs derived heterokaryon referred to movie S11 (scale bar 10 μm). FACS-sorted heterokaryons were plated and after 2 hrs the attached cells were processed for time-lapse imaging for additional 12 hrs. (b) Quantification of heterokaryon cells fate over approximately 12 hrs after sorting. Data represent the total number of heterokaryon analysed (heterokaryons analysed n = 55). (c) Schematic representation of the optimised experimental protocol to study heterokaryon or entotic cell fate.
