Figure 1 | Scientific Reports

Figure 1

From: Cancer derived peptide of vacuolar ATPase ‘a2’ isoform promotes neutrophil migration by autocrine secretion of IL-8

Figure 1

a2NTD treatment stimulates neutrophils polarization and F-actin assembly.

(a) phase contrast images collected after one hour of a2NTD treatment under inverted light microscope (400x magnification). (b) Live cell imaging of neutrophils was performed before and after treatment with either PBS (vehicle control) or a2NTD using FV10i-LIV Laser Scanning Microscope (Olympus). Representative images collected after thirty minutes of treatment from two independent experiments are shown (600x magnification, 1.8x zooming). (c) F-actin assembly in neutrophils was quantified by flow cytometry using phalloidin AF488. Neutrophils were treated with PBS vehicle control, a2NTD or FMLP (positive control). Data were collected from three independent experiments done in duplicates and reported as mean fluorescence intensity (MFI) ± SEM. The statistical significance was compared with control neutrophils, *P < 0.05, **P < 0.01.

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