Figure 2
Telomeric extension inhibition by copper complex 1 in MOLT-4 cells extract.
(A) Electropherogram of telomeric extension inhibition showing ladders generated by the action of telomerase on a TS primer after 33 PCR cycles using conventional TRAP assay with increasing concentrations of complex 1. The lowest band in each lane was TS primer not incorporated for telomere extension. A representative image of four independent experiments is shown. (B) Telomerase activity in MOLT-4 cells measured using real time quantitative PCR (RTQ-TRAP) method. The activity was expressed as the percentage of the activity of the 0 μM solvent control treatment. The telomerase activity was calculated based on Ct values, taking the Ct value as 100% for 0 μM control and 0% when the fluorescence intensity did not reach the fluorescence threshold. Results from four independent experiments are plotted. (C) Immunofluorescence staining analysis of TelC and γ-H2AX foci in MOLT-4 cells (control) and cells treated with 3 μM of complex 1 for 24 h. The dotted white lines indicate the nuclear peripheries. The cell nuclei were stained with DAPI. Scale bar, 5 μm. A representative image of four independent experiments is shown.
