Figure 3 | Scientific Reports

Figure 3

From: Downregulation of oncogenic RAS and c-Myc expression in MOLT-4 leukaemia cells by a salicylaldehyde semicarbazone copper(II) complex

Figure 3

(A) Chemical affinity capture assay using complex 1* against G-quadruplex in MOLT-4 cells. Pulldown DNA samples were PCR amplified to investigate the interaction of complex 1* with KRAS and c-Myc promoters and HTelo. Input was the sonicated DNA fragments after 20 x dilution with ChIP dilution buffer. A representative image of five independent experiments is shown. (B) mRNA levels of c-Myc, c-Kit and KRAS normalized against β-actin, GAPDH and β-tubulin determined by realtime qPCR method. Quantification data are represented as mean ± SD of n = 5. (C) Protein levels of c-Myc, c-Kit and Ras determined using immunoblotting. The initial c-Kit protein level observed was very low even after enriched by membrane protein extraction, and decreased to an almost undetectable level after 24 h. Actin was used as a loading control. The number shown under each protein band indicates the average protein level from four independent experiments. A representative image of four independent experiments is shown.

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