Figure 4
Stable silencing of PTPN3 inhibits colony formation and stemness in resistant ovarian cancer cells.
(A,B) Representative images of the soft agar assay and the quantification of colonies show that stable silencing of PTPN3 inhibited cellular anchorage-independent growth of resistant ovarian cancer cells in vitro. Scale bar = 50 μm. (C,D) Representative flow cytometry plots and the quantification of positive cells show that stable silencing of PTPN3 significantly decreased the ALDH+, CD133+ and ALDH+ CD133+ cell populations. (E,F) Representative images of tumour sphere formation and the quantification of spheres showed that stable silencing of PTPN3 significantly inhibited the sphere formation ability of both cisplatin and doxorubicin resistant ovarian cancer cells. Scale bar = 50 μm. *P < 0.05 (t test).
