Figure 5
The expression of PTPN3 is regulated by miR-199 in resistant ovarian cancer cells.
(A) The predicted binding sites of PTPN3 3′UTR and miR-199 by TargetScan. (B) Luciferase activity in cells co-transfected with miR-199 mimics and the pGL3-PTPN3-wt vector was significantly decreased when compared with the control. (C) Western blotting images showing that overexpression of miR-199 decreased the expression of PTPN3 in resistant ovarian cancer cells. The gels were run under the same experimental conditions. (D,E) The MTS assay showed that overexpression of miR-199 increased the sensitivity of resistant ovarian cancer cells to cisplatin (D) and doxorubicin (E). *P < 0.05 (t test).
