Figure 1

Fanca^−/− mice show moderate impairment of the IgM⁻ to IgM⁺ transition in the BM.

(A) A representative flow cytometry plot used to identify and estimate the frequency of B220⁺ (total B), B220⁺ IgM⁻ CD43^low (pre B) and B220⁺ IgM⁻ CD43^high (pro B) cells isolated from WT and Fanca^−/− mouse BM. (B) Quantification of the data in A (mean ± SEM; n = 5 par genotype). (C) A representative flow cytometry plot used to identify and estimate the frequency of IgM⁻ B220^low and IgM⁺ B220^low populations of BM cells from WT and Fanca^−/− mice. (D) The ratio of IgM⁻ B220^low cells and IgM⁺ B220^low immature B cells identified as shown in C. (n = 8 for Fanca^−/− mice; n = 7 for WT mice *p < 0.05). (E) Flow cytometry representation of B220⁺ IgM⁺ cells derived from WT and Fanca^−/− mouse spleen. (F) Average percentage of B cells (B220⁺ IgM⁻) in the spleen of WT and Fanca^−/− mice (mean ± SEM).