Figure 3
Lung histopathology and P. aeruginosa immunofluorescence staining during chronic infection in resistant and susceptible mice.
Susceptible A/J and resistant C3H/HeOuJ mice were inoculated with 2 × 106 CFU of P. aeruginosa mucoid clinical isolate AA43 embedded in agar beads by intratracheal injection and sacrificed at day 2, 7 and 14 post challenge. The figure exemplifies the lungs of A/J and C3H/HeOuJ mice stained in immunofluorescence with specific antibody against P. aeruginosa (red) (A–D) and with H&E (E–H). Counterstaining for immunofluorescence was performed with 49,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (blue). Bars: 100 μm for immunofluorescence images and 500 μm for H&E images.
