Figure 2 | Scientific Reports

Figure 2

From: Two-Dimensional Gel Electrophoresis-Based Proteomic Analysis Reveals N-terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo

Figure 2

Representative MS/MS identification information of Hsc70 protein spots 3 and 5.

(A) Sequence of the intact Hsc70 protein (coded by transcript GhHsc-3). Peptides identified by MS/MS are labeled with dashed lines: red lines, peptides only identified in spot 3; blue lines, peptides only identified in spot 5; black lines, peptides identified in both spots 3 and 5. The m/z values ([M+H]+) are also indicated under the dashed lines. Numbers represent the calculated m/z for the corresponding peptides, while bracketed numbers represent the observed m/z in our MS/MS experiment. (B,C). Representative peak-view of MS/MS data for the 1680.86 precursor of spot 3 and 2658.36 precursor of spot 5, respectively.

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