Figure 3 | Scientific Reports

Figure 3

From: Two-Dimensional Gel Electrophoresis-Based Proteomic Analysis Reveals N-terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo

Figure 3

Sequence analysis of Hsc70 transcripts.

(A) Multiple sequence alignment of representative Hsc70 proteins from Arabidopsis thaliana (At), Gossypium hirsutum (Gh), Glycine max (Gm) and Oryza sativa (Os). The conserved domains of the Hsc protein family are indicated: with solid lines. (B) Phylogenetic tree of the Hsc70 protein family. Twenty-one members of the Hsc70 family collected from 15 plants were used to construct an NJ phylogenetic tree using Molecular Evolutionary Genetics Analysis (MEGA) software. The bootstrap was set to 1000 replicates. The Hsc70s were divided into four sub-families: cytoplasm, secondary pathway, chloroplast and mitochondrion, which indicated different sub-cellular locations of these Hsc70s. Three GhHsc70 genes cloned from Gossypium hirsutum are labeled in bold. Numbers beside the branches indicate the bootstrap values that supported the adjacent nodes. Ad, Arundo donax; AeTa, Aegilops tauschii; At, Arabidopsis thaliana; Bl, Betula luminifera; Cp, Chimonanthus praecox; Gh, Gossypium hirsutum; Gr, Gossypium ramondii; Gm, Glycine max; Mt, Medicago truncatula; Ob, Oryza brachyantha; Os, Oryza sativa; Pt, Populus trichocarpa; Tc, Theobroma cacao; Vv, Vitis vinifera; Zm, Zostera marina.

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