Figure 4 | Scientific Reports

Figure 4

From: Two-Dimensional Gel Electrophoresis-Based Proteomic Analysis Reveals N-terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo

Figure 4

Quantitative analysis of Hsc70 proteins in cotton ovules and fibers.

(A) Enlarged protein spot 1 and spot 4 of 2-DE maps of WT-0, fl-0, WT-10-O, WT-10-F, WT-10 and fl-10. Spots are marked with arrows in each enlarged 2-DE map. Circles indicate the vanished protein spots. (B,C). Similar information for spots 2 and 6 and spots 3 and 5, respectively. (D–F). Percentages of gray volume (Volume%) for protein spots 1 and 4, protein spots 2 and 6, and protein spots 3 and 5, respectively. (G) Western blot of Hsc proteins in cotton samples of fl-0, WT-0, fl-10, WT-10, WT-10-F and WT-10-O. The symbol + indicates the positive control, which was prokaryote expressed GhHsc70.

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