Figure 5 | Scientific Reports

Figure 5

From: Two-Dimensional Gel Electrophoresis-Based Proteomic Analysis Reveals N-terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo

Figure 5

Edman degradation and Q-TOF analyses of spots 4, 5 and 6.

(A) Edman degradation of protein spots 4, 5 and 6 showed that these proteins share the same 20 N-terminal amino acids, which indicated that Hsc70 may be truncated at 271 amino acids. The twenty sequenced amino acids are bolded. The peptide TACERAK detected by MS/MS and its m/z values (835.39) are indicated. (B) Representative CID MS/MS peak view of the precursor 599.35. The sequence identified was the 465–475 peptide of GhHsc70, with the b and y ions shown. The Y1 ion had a MW of 189.14 kDa, which was 13.98 kDa greater than the theoretical MW of arginine, indicating a potential methylation modification on R475.

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