Figure 1
From: Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans
IgG1 from HEK freestyle produced IgG and IVIg have comparable glycoprofiles.
(a) A schematic model of the bi-antennary glycan as present on IgG Fc at position N297. (b) NanoLC-ESI-MS spectrum of IgG1 produced in HEK cells and (c) human plasma-derived IVIg Kiovig IVIg (data obtained from ref. 37), exhibit a similar glycosylation pattern for the Fc glycan for each direct glycan trait. (d) The similarity is also evident when visualized as calculated derived glycan traits, IgG glycosylation profile of either HEK freestyle produced IgG (this study n = 28) or IVIg (n = 61, data obtained from ref. 37), Error bars indicate standard deviation (SD), **** denotes a statistical significance of p ≤ 0.0001 tested by unpaired t-test.
