Figure 3
From: Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans
Decreasing fucosylation.
(a) The fucose level of IgG1 N297, produced by transfection of IgG heavy and light chain vector in combination with co-transfection of RMD vector. (b) or with addition of 2FF or a combination of 2FF and co-transfection with 5% RMD. (c) Effect of 2FF addition on other derived glycosylation traits (bisection, galactosylation, sialylation). (d) NanoLC-ESI-MS spectrum of IgG produced with 0.4 mM 2FF. The data represents means and SD of three combined independent experiments, * and **** denote a statistical significance of p ≤ 0.05 and p ≤ 0.0001, respectively, as tested by one-way ANOVA using Dunnett’s multiple comparisons test comparing untreated cells with treated. The vertical dotted lines in (b,c) represent the designated optimal concentrations of 2FF.
