Figure 4
From: Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans
Increasing bisecting GlcNAc.
(a) The level of bisecting GlcNAc (bisection) on IgG1 N297 after co-transfection of IgG heavy and light chain vectors with GNTIII vector. (b) The effect of GNTIII co-transfection on other derived glycosylation traits (fucosylation, galactosylation, sialylation). (c) NanoLC-ESI-MS spectrum of IgG produced with 1% GNTIII co-transfection. The data represents means and SD from two independent experiments carried out in an identical fashion, representative of 3 independent experiments *, **, *** and **** denote a statistical significance of p ≤ 0.05, p ≤ 0.01, p ≤ 0.001 and p ≤ 0.0001, respectively, as tested by one-way ANOVA using Dunnett’s multiple comparisons test comparing untreated cells with treated. The vertical dotted lines in (a,b) represent the designated optimal concentrations of GNTIII.
