Figure 5
From: Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans
Decreasing galactosylation.
(a) The galactosylation level of IgG1 N297 produced with addition of 2FG. (b) Effect of 2FG addition on other derived glycosylation traits (fucosylation, bisection, sialylation). (c) NanoLC-ESI-MS spectrum of IgG produced with 1 mM 2FG. The data represents means and SD of four combined independent experiments, *, **, *** and **** denote a statistical significance of p ≤ 0.05, p ≤ 0.01, p ≤ 0.001 and p ≤ 0.0001, respectively, as tested by one-way ANOVA using Dunnett’s multiple comparisons test comparing untreated cells with treated. The vertical dotted lines in (a,b) represent the designated optimal concentrations of 2FG.
