Figure 5

PA regulates adipogenic differentiation by displacing DEPTOR from mTORC1.

(a) 3T3-L1 preadipocytes were transfected with a PPRE luciferase reporter plasmid vector, induced to differentiate for 3 h, and then luciferase activity was measured. (b) 3T3-L1 cells were differentiated in the absence or presence of 300 μM C8-PA. Cell lysates and immunoprecipitates against raptor were analyzed by western blotting. (c,d) 3T3-L1 cells were infected by either shscramble or shDEPTOR and selected with puromycin for five days. After eight days of differentiation with or without 300 μM C8-PA, cells were subjected to either (c) Oil Red O staining and quantification of lipid content, or (d) western blotting. (a–d) All data shown are mean ± SD or are blots representative of three to five independent experiments. Student t-tests were performed to compare the indicated pairs of data. *P < 0.05.