Figure 6
From: PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639
PA inhibits adipogenesis through the IRS-1-Akt axis.
(a) 3T3-L1 preadipocytes were serum-starved overnight, then stimulated with 300 μM C8-PA for 30 min. Cell lysates were analyzed by western blotting. (b) 3T3-L1 cells were induced to differentiate either with or without 300 μM C8-PA for eight days, then lysed and subjected to western blotting. (c) 3T3-L1 cells were infected with either adGFP or adPLD1, induced to differentiate for eight days, and then analyzed by western blotting. (d) Pld1+/+ and Pld1−/− MEFs were differentiated for eight days, lysed and analyzed by western blotting. (e) Cells were infected with either shscramble or shPLD1 #1, selected with puromycin for five days, then induced to differentiate either with or without 300 μM PA for eight days, followed by analysis by western blotting. (f) 3T3-L1 cells were infected with either shscramble or shDEPTOR and treated either with or without 300 μM PA for 30 min, then lysed and analyzed by western blotting. (a–f) All data shown are blots representative of three to five independent experiments.
