Figure 2 | Scientific Reports

Figure 2

From: MiR-101 Targets the EZH2/Wnt/β-Catenin the Pathway to Promote the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

Figure 2

miR-101 promotes the osteogenic differentiation of hBMSCs.

(A) miR-101 mRNA expression in hBMSCs infected by miR101-lentivirus or transfected with anti-miR101 was analyzed by qRT-PCR with U6 as a control before osteogenic differentiation. (B) The osteoblastic marker genes RUNX2, ALP, OPN and OCN were analyzed by Western Blot at day 15 of osteogenic differentiation with β-actin as a control. (C) ALP activity was measured at day 15. (D) Alizarin Red staining was measured at day 15 and quantification is shown at right. All of the data are expressed as the mean ± SD. *p < 0.05, **p < 0.01.

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