Figure 4 | Scientific Reports

Figure 4

From: MiR-101 Targets the EZH2/Wnt/β-Catenin the Pathway to Promote the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

Figure 4

EZH2 mediates the miR-101-regulated osteogenic differentiation of hBMSCs.

(A) EZH2expression was examined at different time points during osteoblast differentiation by Western blot. (B) The expression of EZH2 was assessed by Western blot. (C,D) EZH2 overexpression inhibits the expression of osteoblastic marker genes and reduces the ALP activity in miR-101-overexpressing cells. (E) Alizarin Red staining was measured and quantification is shown at right. (F) Up: ALP activity was measured; down: EZH2 expression was analyzed by Western blot. All of the data are expressed as the mean ± SD. *p < 0.05, **p < 0.01.

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