Figure 1

Treatment schedules and overview of the ssRNA-Seq data.

(A) The schematic timeline of METH treatment regimen. Habituation (day (−7)–(−1)): The mice were handled daily for 1 week to adapt to the experimenter before treatment. Pre-test (day 1-2): mice were given once daily injections of saline for two consecutive days. Development (day 3–7): After pre-test, mice were divided into two groups randomly, and were given once daily injections of METH (2 mg/kg) or saline for five consecutive days. Transfer (day 8–9): two groups of mice were experienced two injection-free days. Expression (day 10): mice were given a challenge injection of either 2 mg/kg dose of METH or saline. Horizontal locomotor activity was performed 60 minutes before and after the injections on all drug treatment days. (B) Statistical alignment of the sequencing data from the saline and METH groups. Unique mach represents the mapped reads that aligned to only one position in the mouse genome. Multi-position mach represents the mapped reads that aligned to more than one positions in the mouse genome. Total unmapped indicates reads that did not match to the mouse genome.