Table 1 Activation of the terminators of Nab6p-binding mRNA genes by overexpression of NAB6 and PAP1.

From: Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae

Gene

Activation by NAB6 + PAP1 (-fold)a

Candidate for cis-elements

Scoreb

Terminator activityc

ASP3*

1.3

GTTCt

9.1

2.28

PIR1*

1.5

GTTCt

7.0

0.26

FMP52

1.6

GTTCG

5.2

1.92

MFA1

1.9

GTTCG

4.5

0.49

SCW4*

1.6

GTTCG

4.4

2.30

TIR2*

1.6

GTTCG

4.3

1.85

YDR133C

1.5

GTTCG

4.3

--

RPL26B

-

--

3.8

1.30

RPL26A

-

GTTCG

3.7

0.95

HSP150*

1.5

GTTCt

3.7

0.33

GTTCG

YLR285C-A

-

--

3.6

0.09

SUC2

-

--

3.5

1.04

GPB1

1.3

GTTCG

3.4

0.17

YGP1*

1.6

GTTCG

3.2

0.99

SUI1

-

--

3.1

1.73

YDL159W-A

-

--

2.9

0.11

DDR2

1.6

GTTCG

2.7

0.34

YPR053C

-

--

2.6

--

DIT1

2.9

GTTCG

--

2.22

  1. -, no significant activation (<1.2-fold; see Extended Data Fig. 8).
  2. --, not detected.
  3. *Cell wall–related genes (Saccharomyces Genome Database); http://www.yeastgenome.org/).
  4. aRelative intensity of GFP fluorescence of each terminator strain harboring pGP564-NAB6-PAP1 compared with that of the corresponding terminator control strain harboring pGP564 vector.
  5. bThese scores are index values for the interaction between Nab6p and the corresponding mRNA (from ref. 24).
  6. cActivity relative to that of the standard PGK1 terminator (from ref. 2).
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