Figure 3
From: TRPC3 positively regulates reactive oxygen species driving maladaptive cardiac remodeling
TRPC3 forms a stable ternary complex with Nox2 and p22phox.
(a,b) Expression of Nox2 and p22phox proteins in HEK293 cells that express a different combination of TRPC3-GFP and GFP. Results of a quantitative analysis are shown in (b) (n = 3). (c) Nox2 mRNA amounts in HEK293 cells co-expressing Nox2 with GFP or TRPC3-GFP (n = 3). (d) Increased Nox2 and p22phox protein in HEK293 cells co-expressing pore-dead mutant of TRPC3 (n = 3). (e) Interaction of TRPC3 with Nox2 in HEK293 cells. Immunoprecipitation was performed using an anti-flag antibody. (f) Nox2 protein expression in HEK293 cells expressing TRPC3 alone or co-expressing TRPC3 and TRPC6 (n = 3). Error bars, s.e.m. *P < 0.05, **P < 0.01.
