Figure 4
From: TRPC3 positively regulates reactive oxygen species driving maladaptive cardiac remodeling
TRPC3 forms a stable ternary complex with Nox2 and p22phox proteins in endogenously p22phox -absent CHO cells.
(a) Expression of Nox2 and p22phox proteins in CHO cells that express a different combination of TRPC3-GFP and GFP. (b) Results of quantitative analysis (n = 3). (c) Expression of Nox2 and p22phox co-expressed with either GFP or TRPC3-GFP in MG132 (10 μM)-treated CHO cells. (d) Graphs depict the relative expression of either Nox2 or p22phox protein to that in non-treated cells. Band intensities were normalized by GAPDH. (e–g) Interaction of TRPC3 with p22phox and Nox2 in CHO cells. (h) Localization of Nox2 in CHO cells co-expressing Nox2 with TRPC3-GFP (or GFP-F). Error bars, s.e.m. *P < 0.05, **P < 0.01.
