Figure 1 | Scientific Reports

Figure 1

From: Aberrant SSEA-4 upregulation mediates myofibroblast activity to promote pre-cancerous oral submucous fibrosis

Figure 1

Up-regulation SSEA-4 expression in OSF.

(a) Normal buccal mucosa (N) tissues and fibrotic buccal mucosa (F) lesions were subjected to real-time RT-PCR analysis for the determination relative expression levels of ST3GAL2. (b) Real-time RT-PCR analysis demonstrated that ST3GAL2 expression in independent pairs (n = 5) of normal human buccal mucosal fibroblasts (BMFs) and human fibrotic buccal mucosal fibroblasts (fBMFs). (c) Positive correlations between SSEA-4 and α-SMA in OSF tissues were analyzed. (d) Representative images of normal buccal mucosa (N) tissues and OSF specimen lesions were subjected to histological analysis for the expression levels of SSEA-4. (e) BMFs were serum-starved (0.5% FBS) for 48 h and treated with indicated concentrations of arecoline for further 24 h in serum-free medium. The SSEA-4 activity in arecoline-treated BMFs was assessed by flow cytometry and presented as relative fold-changes. *Represents significant difference from control values with p < 0.05.

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