Figure 5

Depletion of ST3GAL2 repressed arecoline-induced myofibroblastic differentiation activity in BMFs.
(a) The silencing effect of ST3GAL2 shRNA in arecloine-treated BMFs was validated by flow cytometry. Single cell suspension of arecoline-treated BMFs infected with ST3GAL2-specific shRNA or control sh-Luc lentivirus was analyzed for collagen gel contractility (b), migration ability (c), and wound healing ability (d). *P < 0.05 Sh-Luc.+arecoline group versus Sh-Luc. group; #P < 0.05 Sh- ST3GAL2+arecoline or Sh-2+arecoline versus Sh-Luc.+arecoline group.