Figure 1
Replicative senescent mouse fibroblasts are resistant to VSV infection.
(A) Growth curve of serially-passaged MEFs showing accumulated population doublings (PDLs) with time. (B) Microscopy images of serially-passaged MEFs showing morphology (left panels) and SA-beta-gal staining (right panels) of early passage (upper panels) and late passage senescent (bottom panels) MEFs. Quantification of the SA-beta-gal positive cells is shown below (at least 200 cells were counted per condition). (C) Viral titers (PFU/mL) determined in early or late passage senescent MEFs after the indicated periods of infection (hours post infection, hpi) at a multiplicity of infection (MOI) of 0.05 PFU/cell. (D) Western-blot analysis of VSV protein synthesis in early or late passage senescent MEFs after the indicated periods of infection at MOIs of 0.05 PFU/cell (upper panel) or 10 PFU/cell (lower panel). Actin is shown as loading control. (E) Percentage of apoptotic cells measured after mock or VSV infection at MOI of 10 PFU/cell, in early or late passage senescent MEFs. Data are mean values +/− SE from at least three different experiments. *p < 0.05, **p < 0.01, ***p < 0.001 Student’s t test.
