Figure 1

Sampling of materials and ranking of candidate reference genes.
(a) Samples representing different developmental stages of fruits for sequencing. (b) Comprehensive ranking of candidate reference genes based on their expression stability in all 14 samples calculated by geNorm. Stage 2 represents the flowering stage. Stages 3, 4 and 7 represent samples 18, 31 and 40 days after flowering, respectively. Scale bar = 1 cm. The y-axis represents the expression stability of gene based on normalization M. Mthreshold < 1.5 for solo calculation.