Table 1 Characteristics of candidate reference genes.

From: LbCML38 and LbRH52, two reference genes derived from RNA-Seq data suitable for assessing gene expression in Lycium barbarum L.

Gene ID

Gene abbreviation

Mean raw fragments

Standard Deviation (SD)

Coefficient of variation (CV)

Swissprot annotation

CL10201.Contig1_All

LbCYP

1142.214286

718.6318388

0.62915676

Peptidyl-prolyl cis-trans isomerase CYP20-2, chloroplastic OS = Arabidopsis thaliana GN = CYP20-2 PE = 1 SV = 1

CL1082.Contig3_All

LbTBP

138.5714286

38.5141618

0.27793725

TATA-box-binding protein OS = Solanum tuberosum GN = TBP PE = 2 SV = 1

Unigene36249_All

LbGAPDH

145.4285714

68.28527748

0.46954513

Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic OS = Arabidopsis thaliana GN = GAPC1 PE = 1 SV = 2

CL13903.Contig10_All

LbTUB

57.57142857

57.88412069

1.005431377

Tubulin beta-2 chain OS = Solanum tuberosum GN = TUBB2 PE = 2 SV = 1

CL2539.Contig4_All

LbEF1α

3196.071429

823.6067456

0.257693473

Elongation factor 1-alpha OS = Solanum lycopersicum PE = 2 SV = 1

CL4826.Contig3_All

LbActin7

626.5714286

191.7922411

0.306097968

Actin-7 OS = Arabidopsis thaliana GN = ACT7 PE = 1 SV = 1

Unigene44516_All

LbHSP90

126.0714286

53.97501457

0.428130427

Heat shock protein 90–2 OS = Arabidopsis thaliana GN = HSP90-2 PE = 1 SV = 1

CL13810.Contig1_All

LbHIS3

2307.928571

612.946536

0.265582975

Histone H3.3 OS = Vitis vinifera PE = 2 SV = 3

CL10058.Contig1_All

LbCYC

1009

92.22297394

0.091400371

Cyclin-B1-5 OS = Arabidopsis thaliana GN = CYCB1-5 PE = 2 SV = 3

Unigene55248_All

LbEIF4A

2087.928571

165.8161467

0.07941658

Eukaryotic initiation factor 4A-3 OS = Nicotiana plumbaginifolia PE = 2 SV = 1

Unigene19346_All

LbPP2A

831.8571429

99.63923937

0.119779268

Serine/threonine-protein phosphatase PP2A catalytic subunit OS = Nicotiana tabacum PE = 2 SV = 1

CL11175.Contig21_All

LbUBQ

1211.428571

186.5371542

0.153981141

Polyubiquitin 10 OS = Arabidopsis thaliana GN = UBQ10 PE = 1 SV = 2

Unigene15131_All

LbCML38

695

106.1066227

0.1526714

Calcium-binding protein CML38 OS = Arabidopsis thaliana GN = CML38 PE = 2 SV = 1

CL7969.Contig1_All

LbLEA

525.7142857

113.3995174

0.215705604

Late embryogenesis abundant protein Lea14-A OS = Gossypium hirsutum GN = LEA14-A PE = 2 SV = 1

Unigene31075_All

LbEF1β

5663

1138.411849

0.201026285

Elongation factor 1-beta OS = Oryza sativa subsp. japonica GN = Os07g0662500 PE = 1 SV = 3

Unigene26461_All

LbSKIP

612.5

120.2361459

0.196303912

F-box protein SKIP31 OS = Arabidopsis thaliana GN = SKIP31 PE = 1 SV = 1

Unigene55138_All

LbRH52

950.4285714

113.8816621

0.119821379

DEAD-box ATP-dependent RNA helicase 52 OS = Arabidopsis thaliana GN = RH52 PE = 2 SV = 1

Unigene59903_All

LbRPL7A

793.6428571

149.1166849

0.187888902

60S ribosomal protein L7-1 OS = Arabidopsis thaliana GN = RPL7A PE = 2 SV = 1

Unigene59939_All

LbMYB1

2982.571429

697.3143085

0.233796348

Transcription factor MYB1R1 OS = Solanum tuberosum PE = 2 SV = 1