Table 2 Primer sequences and amplicon characteristics of the 18 reference genes.

From: LbCML38 and LbRH52, two reference genes derived from RNA-Seq data suitable for assessing gene expression in Lycium barbarum L.

Gene abbreviation

Forward primer (5′-3′)

Reverse primer (5′-3′)

TM (°C)

Amplicon size (nt)

PCR efficiency (%)

Correlation coefficient (R2)

Mean CT

Standard Deviation (SD)

Coefficient of variation (CV)

LbCYP

TCGTTGCGTCTGGCTACTTCA

CTGTCTGCGGCACATCATCAC

61

208

91.3

0.9953

20.19

2.166658889

0.107307139

LbTBP

CGACGAATGGCAGATCAAGGATA

CAAGTTCACCGTTGAGACAATGTT

62

108

101.0

0.9937

24.67

2.140584019

0.086744429

LbGAPDH

CACGGTCAATGGAAGCACAAT

GCAGCAGCCTTGTCTTTATCC

60

179

94.8

0.9775

22.46

2.267361358

0.100925407

LbTUB

GTCCAGAACAAGAACTCGTCCT

CGCCCTCCTCATCATACTCCT

60

325

96.1

0.9747

23.58

2.294896689

0.097316984

LbEF1α

TCGTGTGGAGACTGGTGTAATC

TCGCCTGTCAATCTTGGTCAA

60

352

92.2

0.9973

17.43

2.401203192

0.137725035

LbActin7

GGTCCTCTTCCAGCCATCCAT

TGAGCCACCACTGAGCACAA

62

133

90.6

0.9962

20.29

1.841419014

0.090747549

LbHSP90

TCCTGATAGTCCTGCTGAGTTGG

TTCCGTTGATGCTTCTGCTGATG

60

121

97.3

0.9502

18.55

2.043184756

0.110139076

LbHIS3

CACTACAGGTGGTGTGAAGAAG

CACGAACAAGCCTCTGGAAG

61

128

91.9

0.9957

17.18

1.561123661

0.090835934

LbCYC

TTCTTGTCATACTCGGTGTTGTGT

CTGAAGTTGTCTCTGTGCCTGTA

60

119

98.5

0.9977

21.01

1.875417615

0.089258037

LbEIF4A

ACGGAGATATGCCACAGAAGGAG

AGAGCGACCAATGCGATGAATG

60

182

92.2

0.9543

20.79

1.830072065

0.087990277

LbPP2A

GAGATGCTGTGAAGAGATGGTGAA

AGAAGATTACACGAACGCTCATTGA

61

246

105.0

0.9857

21.17

1.30967704

0.061843202

LbUBQ

GGCATTCCTCCAGATCAGCAA

GTGTCCGAACTCTCAACCTCAA

60

291

106.1

0.9993

20.14

2.090803559

0.103768094

LbCML38

CGGTGGTTCTTCTGGTTCATCAA

TCTTCTTGTGCCTCCTCATTAACTC

62

192

105.7

0.9958

21.68

1.708817146

0.078819113

LbLEA

TGTCTGGCTCTCCGATGTCAA

TCTCCAACTTCAATCCTCATCTTCA

61

310

92.6

0.9851

18.37

1.741954661

0.094816225

LbEF1β

GACGATGACGACGACGACAT

GCGAACAGCCTCCTCAAGT

60

186

94.9

0.9947

18.88

1.674620435

0.088683577

LbSKIP

TGAGGAGGAGGAGGAGGAAGAA

TGAGGATGTGACGGAGCAACT

60

293

102.4

0.9936

25.02

2.10058639

0.083924345

LbRH52

GCAGGCAAGTCAGGATTAGCA

CGCATAACGAGTCAACCATTCAG

62

123

97.5

0.9972

22.02

1.636191649

0.074287127

LbRPL7A

CCTTTACTCTCACCAAGCCAGAA

TCACAACTACGATACACCGAACA

60

162

110.1

0.9536

20.99

1.774507767

0.084515702

LbMYB1

TCCGCCGAAGCAACCTCAAC

CATAGGAACAGGACGAACCAGCAT

60

290

108.2

0.9809

21.11

3.398526812

0.16096409

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