Figure 1 | Scientific Reports

Figure 1

From: Photothermal therapy improves the efficacy of a MEK inhibitor in neurofibromatosis type 1-associated malignant peripheral nerve sheath tumors

Figure 1

PD901 effectively treats MPNSTs in vitro by blocking ERK activation.

(a) Mouse MPNST (M2) cells exhibit markedly decreased p-ERK protein expression when treated with 1 μM PD901 for 4 h or 8 h compared to vehicle (DMSO)-treated M2 cells when visualized by a Western blot. (b) M2 cells exhibit decreased viability when treated for 48 h with increasing concentrations of PD901 (IC50 = 1 μM). (c) M2 cells treated with 0.1, 1, and 10 μM PD901 for 24 h undergo cell death via both apoptosis (light blue, Annexin V + 7AAD- population) and late apoptosis/necrosis (dark blue, 7AAD + population), measured by flow cytometry. (d) M2 cells treated with 0.1, 1, and 10 μM PD901 for 24 h undergo cell cycle arrest in the G0/G1 phase in a concentration-dependent manner, measured by flow cytometry. Data in all plots expressed as mean ± standard deviation (n ≥ 3/group); *p < 0.05; **p < 0.01; ***p < 0.001.

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