Table 1 The oligonucleotide primer sequences and PCR conditions for β-catenin and cyclin D1.
Gene | Primer sequence | PCR condition |
|---|---|---|
β-catenin | 5°-CGGGATCCACAAGAAACGGCTTTCA-3° (sense) and 5°-GAGAATTCCAGGTCAGTATCAAACCA-3° (antisense). | 94 °C for 3 minutes, followed by 35 cycles at 94 °C for 30 seconds, 55 °C for 1 minute, and 72 °C for 1 minute. |
Cyclin D1 | 5°-CTGGCCATGAACTACCTGGA-3° (sense) and 5°-GTCACACTTGATCACTCTGG-3° (antisense). | 94 °C for 3 minutes, followed by 35 cycles at 94 °C for 30 seconds, 60 °C for 20 seconds, and 72 °C for 45 seconds. |
