Figure 2
From: Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration
Calpain-inhibition attenuates acute axonal degeneration in the optic nerve in vivo.
(a) AAV.EGFP was injected intravitreally to label the axons of retinal ganglion cells. After two weeks, calpeptin in 7% DMSO or 7% DMSO as control was injected intravitreally 2.5 h before optic nerve crush. Single axons in the optic nerve were imaged within 400 μm from the lesion site before crush and up to 6 h after crush. (b,c) Representative images of axonal changes proximal (b) and distal (c) to the crush site at the indicated time points after crush and previous calpeptin treatment compared to controls. (d,e) Quantification of axonal integrity ratios proximal (d) and distal (e) to the crush site at the indicated time points after crush after calpeptin treatment compared to control. The axonal integrity ratio is the sum of the lengths of the axon fragments at a given time-point divided by the initial length of the axon. 5–6 animals per group. Error bars represent the standard error of the mean (SEM). *P < 0.05, ***P < 0.001 by independent samples t-test.
