Figure 6
From: Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration
Effects of CRMP2 overexpression on crush-induced AAD in vivo.
(a) Intravitreal injections of AAV.mcherry or AAV.CRMP2 visualized the axons of retinal ganglion cells. After four weeks, single axons within 400 μm of the crush site were imaged before crush and during 6 h after crush. (b,c) Representative images of lesioned axons proximal (b) and distal (c) to the crush site at the given time points after crush and previous transduction with either AAV.CRMP2 or AAV.mcherry (control). In the control group (upper row), a rapid formation of degeneration bulbs and subsequent axonal fragmentation can be observed. These morphological hallmarks of AAD are significantly less pronounced in the axons overexpressing CRMP2 (lower row). (d,e) Quantification of the axonal integrity ratio proximal (d) and distal (e) to the crush site at the indicated time points after crush. 5–7 rats per group. Error bars represent the standard error of the mean (SEM). **P < 0.01, ***P < 0.001 by independent samples t-test.
