Figure 4 | Scientific Reports

Figure 4

From: Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways

Figure 4

BafA1 inhibits autophagy flux in HCC cells.

(A) BEL7402 and HepG2 cells were treated with vehicle control or with 5 nM BafA1 for 24 h, and analyzed by transmission electron microscopy (TEM). Typical structures enclosed in the black square have been enlarged in the right panel for more detailed imaging. (B) Cells were also treated as above (A), and protein expression was examined using IB analysis for LC3, p62 and β-actin. (C) Following transfection of BEL7402 and HepG2 cells with GFP-LC3 for 24 h, cells were treated for 4 and 24 h. The numbers of cells with GFP-LC3 punctate are represented. (D) BEL7402 and HepG2 cells were treated for 24 h following transfection with GFP-mRFP-LC3. Images were captured by confocal microscopy (60 × oil immersion lens), scale bar = 25 μm. Data are presented as mean ± SEM from three independent experiments, (BafA1 means Bafilomycin A1).

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