Figure 5 | Scientific Reports

Figure 5

From: Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways

Figure 5

Inhibition of autophagy induction decreases BafA1-mediated cell death.

(A) BEL7402 and HepG2 cells were vehicle-treated or treated with 5 nM BafA1 for 24, 48, 72 h. Protein expression of p-Akt, Akt, p-mTOR, mTOR, p-p70S6K and p70S6K were analyzed by IB, using β-actin as a loading control. (B) Cell lysates reprsenting BEL7402/shLC3, HepG2/shLC3 or BEL7402/shControl and HepG2/shControl cells were analyzed by IB to determine the efficiency of LC3 knockdown. (C) BEL7402/shLC3, HepG2/shLC3 or control cells (BEL7402/shControl and HepG2/shControl) were treated with vehicle control or 5 nM BafA1 for 24, 48 and 72 h, after which time cell viability was determined using the MTT assay. Data are presented as mean ± SEM from three independent experiments, (BafA1 means Bafilomycin A1).

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