Figure 1
From: Single GDP-dissociation Inhibitor Protein regulates endocytic and secretory pathways in Leishmania
Cloning of LdGDI from Leishmania donovani.
(a) 1338 bp fragment (lane 2) was amplified from L. donovani cDNA by PCR using appropriate forward and reverse primers as described in Methods. Lane 1 represents DNA ladder. The sequence of LdGDI was submitted to GenBank database under accession no. KX181889. (b) Hypothetical translation of the obtained sequence into protein revealed that LdGDI shows significant similarity with Rab-GDI from B. Taurus, S. cerevisiae, P. falciparum and G. intestinalis. Residues corresponding to 5 characteristic conserved or SCR of Rab-GDI are marked with line and the consensus sequences in SCRs of GDI are indicated in Bold. (c) Phylogenetic comparison of LdGDI with GDI from different organisms. The dendrogram was generated by Jalview program based on percent identity. (d) Putative 3D structure of LdGDI based on homology-modeling using SWISS-MODEL automated protein structure server. SCR 1 and 3B are shown in orange, SCR 3A in purple and SCR 2 in green colour. Residues found in Rab-binding platform and mobile effector loop are shown in red sticks.
