Figure 6
From: Single GDP-dissociation Inhibitor Protein regulates endocytic and secretory pathways in Leishmania
Role of LdGDI in the secretory pathway in Leishmania.
(a) To determine the role of LdGDI in the secretory pathway in Leishmania, we determined the levels of cell-associated and secreted gp63 by respective L. donovani promastigotes by western blot analysis using specific antibodies as described in Methods. Full length blot is presented in Supplementary Fig. 4. Results are representative of three independent experiments. (b) Quantitative analysis of amount of gp63 present in the cell lysate and secreted by indicated parasites. Amount of gp63 present in the cells and secreted by vector control cells were taken as 100 Unit. Results are expressed as mean ± SD.
