Figure 4
From: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
TMED3 promotes cell migration by increasing IL-11 expression.
(A) Differentially expressed genes in HepG2-siTMED3 cells compared with control cells. The top 10 up- and down-regulated genes are listed on the right. (B,C) Relative IL-11 expression in cells with stable TMED3 knockdown or overexpression and paired controls. IL-11 expression was analyzed by western blot and normalized to β-actin. (D,E) The IL-11 concentration in culture medium from cells with stable TMED3 knockdown or overexpression and paired controls was determined by ELISA. n = 3, mean ± SD, the Student’s t test, *p < 0.05 versus GFP control, **p < 0.01 versus GFP control. (F,G) Relative P-STAT3 and STAT3 expression levels in cells with stable TMED3 knockdown or overexpression and paired controls were analyzed by western blot and normalized to β-actin. (B–G) n = 3.
